Earlier isolation and identification of salmonella from clinical samples by traditional cultural techniques required laborious procedures which can last upto 7 days whereas amplification of dna sequences unique to an organism using the pcr improves both the speed of detection and the level of sensitivity at which organisms can be detected and has been increasingly used to identify several bacterial species from food and clinical samples. Molecular based detection of salmonella spp delivers one of the fastest times to result with run times after enrichment and dna extraction of 45 min to 3h the predominant molecular approach is polymerase chain reaction pcr that detects dna regions specific to salmonella commercial pcr detection kits are usually in a user friendly format . Loop mediated isothermal amplification lamp has become a powerful alternative to polymerase chain reaction pcr for pathogen detection in clinical specimens and food matrices nontyphoidal salmonella is a zoonotic pathogen of significant food and feed safety concern worldwide. Since nucleic acids and nonviable cells are detected by pcr microbiologists are concerned with the significance of a pcr signal in addition pcr which is based on dna detection is not able to discriminate between dead and viable cells for salmonella in meat we claim that dead cells or free nucleic acids do not play a major role
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